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For the inducible experiments, mice were pre-administered doxycycline hyclate at a concentration of 2 mg/mL in their drinking water starting one week prior to intravenous injection of cancer cells as described above, and treatment was discontinued three days post-injection

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Interestingly, the standard mutant T-p53C-Y220C showed a negligible shift (ΔT m = 0.3 °C)