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Aliquots of the cDNA were used to measure the expression levels of the genes with the primers, and Power SYBR Green master mix (Applied Biosystems, ThermoFisher Scientific) on a 7900 HT sequence detection system (Applied Biosystems, ThermoFisher Scientific)
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Transcription of the insulin gene to preproinsulin mRNA is sophisticated and reflects the tight regulation by transcription factors and recruited coactivators
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