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As example, an analytical approach for the determination of S-glutathionylated proteins based on the specific reduction of mixed disulfides by Grx, their reaction with N-ethylmaleimide–biotin, followed by affinity purification on avidin-agarose of tagged proteins and identification by proteomic analysis has been developed [129]
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The commonly studied oral glutathione dosage ranges from 250 to 500 mg per day
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