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25 Additionally, a small prospective study of 12 transgender individuals, aged 17-33 years, showed a significant decrease in endothelin levels after 4 months of GAHT (8.1 ± 3.0 to 5.1 ± 2.0 ng/mL
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This procedure involved removing the supernatant and resuspending the mitochondrial pellet in an isolation buffer of 250 mM sucrose, 0.5 mM EDTA, and 10 mM Tris-HCl to achieve a final protein concentration of 20-25 mg/ml [2]
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