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Furthermore, the apoptosis of SGNs detected by C-CASP3 immunostaining and western blot showed that there was much less C-CASP3-positive SGNs and remarkable reduction of the C-CASP3 protein levels in the NAC co-treated groups compared with the peroxynitrite-only groups, and it was also lower in the peroxynitrite + gpx1 –/– + NAC group compared with the peroxynitrite + gpx1 –/– group (Figures 7B,C)
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