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Following blocking, the slides were incubated overnight at 4°C in a moist chamber with primary antibodies against trimethyl-Histone H3 (Lys4) (H3K4me3, rabbit polyclonal antibody, Merck #05-1339) and trimethyl-Histone H3 (Lys9) (H3K9me3, rabbit polyclonal antibody, Merck #07-442), diluted at 1:800 and 1:2000, respectively, in Rinse Buffer (EMD Millipore) plus 0.1% Tween 20 (Sigma-Aldrich)

Canadian

Dosage:5/5mg (standard)

Brain histology