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However, the specific knockout of KLF4 in AdvSca1-SM cells induces it to differentiate into a more protective cell phenotype, and enhances plaque stability by changing plaque composition (such as reducing necrotic core and increasing fiber cap thickness), thus inhibiting atherosclerosis from developing toward instability (106)

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Moderate caloric restriction (15-20% below maintenance) supports AOD-9604 protocols without triggering metabolic adaptation or excessive muscle loss