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or cell suspensions were first centrifuged at 400 g for 3 minutes, then pellets were gently resuspended in their respective thaw media, and then cell viabilities were determined (with centrifugation

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Ligands enable receptor-mediated transcytosis (RMT), allowing nanoparticles to "hitchhike" across endothelial cells by binding to specific receptors expressed on the BBB surface