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In the present study, the similarity of ACh-induced dilation of MCA of Protandim-treated and nontreated rats fed low salt diet and the similar ACh-induced dilations of MCA from wild type and Nrf2 (−/−) knockout rats fed low salt diet indicate that NRF2 mechanisms in the vasculature are not strongly active during normal physiological conditions, and that activation of NRF2 under those conditions has no deleterious effects