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Altoé PM et al
cat_infiniwell
ML:30 ml
The lysates were separated on 8–12% polyacrylamide gels by electrophoresis, transferred onto nitrocellulose membranes, and probed with specific primary antibodies targeting the following proteins: p-AKT (1:1000, Cell Signaling Technology, USA, 9271), total AKT (1:1000, Cell Signaling Technology, USA, 9272), PI3K (1:1000, Cell Signaling Technology, USA, 4249), Bax (1:1000, Cell Signaling Technology, USA, 2772), Bcl-2 (1:2000, Abcam, UK, ab182858), IGF-1R (1:1000, Abcam, UK, ab182408), IGF-2 (1:1000, Abcam, UK, ab177467/ab317490), IGFBP-3 (1:1000, Abcam, UK, ab272857), IGFBP-6 (1:1000, Abcam, UK, ab219560), Caspase-3 (1:1000, Cell Signaling Technology, USA, 9662), Cleaved Caspase-3 (1:1000, Cell Signaling Technology, USA, 9661), and Caspase-9 (1:1000, Cell Signaling Technology, USA, 9504)
