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The BBB was realized by co-culturing ECs in a microfluidic channel (“vascular microchannel”) under continuous perfusion with ACs and PCs embedded in a hydrogel matrix (“brain compartment”) 25 ECs and PCs were seeded on opposite sides of a porous membrane to form monolayers and ensure close proximity between the cells
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Over time, these changes can reinforce isolation, making it harder to reconnect
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